Doctoral Research Associate (Wissenschaftliche*r Mitarbeiter*in , E 13 TV-L, 65%)

This DFG-funded project aims to develop a molecular understanding of the role of the conformational dynamics of the translation initiation factor eIF4A for the efficiency of mRNA translation and gene expression within the functional network of translation initiation and protein biosynthesis. Work in this project involves.

• the recombinant production of translation factors in E. coli
• activity assays
• the preparation of RNA by in vitro transcription
• the determination of translation efficiencies in in vitro reporter assays
• the planning, execution and analysis of single-molecule FRET experiments
• the presentation of results in internal seminars and at conferences

Required:

• a university degree (MSc) in biochemistry, chemistry, biology or a related subject
• knowledge and experience in molecular biology techniques such as cloning and mutagenesis
• knowledge and experience in the recombinant production of proteins and their purification by chromatographic techniques
• a strong command of English, both written and spoken
• the ability to work both independently and as part of a team
• a genuine interest in research

Desirable:

• previous experience in the field of single-molecule microscopy
• experience with RNA preparation by in vitro transcription

• We provide thorough training in modern biochemical and biophysical techniques.
• Your mentoring will be tailored to your individual needs and goals.
• You will have the opportunity to participate in national and international conferences.
• You will be integrated into a dynamic, international and interdisciplinary team.
• Appreciation, commitment, openness and respect – values which are important to us.
• Our broad range of diverse work-time models offers great flexibility – also when working from home.
• If you have family members or young children in your care, our Family Service Office offers concrete support to help you balance your private and professional responsibilities.
• As an educational institution, we are deeply committed to offering occupational training and continuing education opportunities tailored to your individual needs. -
• From A – Z, Aikido to Zumba, our sport and health programmes ensure a healthy work-life balance.
• As a university employee, you are entitled to numerous benefits afforded to public servants, e.g. an attractive company pension scheme (VBL), an annual end-of-year bonus and a position that is shielded from economic fluctuations